Features of structure and dynamics in damages of fertile properties in ejaculate under the influence of toxocariasis invasion
DOI:
https://doi.org/10.34287/MMT.4(47).2020.2Abstract
Purpose of the study. To study the features of changes of spermiologic values and degree of spermatozoa DNA fragmentation depending on the presence/absence of toxocariasis invasion.
Materials and methods. 89 men aged 20 to 45 years were examined, which were divided into 5 groups. The first (control) group was 12 fertile men; the second group (comparison group) – 27 infertility patients with normal level of DNA fragmentation of sperm and without antibodies to toxocariasis; The third group – 20 infertility men with normal level of DNA fragmentation of sperm and presence of antibodies to toxocariasis. By the fourth and fifth groups included 15 infertility men with high levels of DNA fragmentation of sperm and the presence of antibodies to toxocariasis and lack of respectively. A comprehensive research was conducted for all men that included analysis of spermogram according to WHO recommendations, determination of sperm DNA fragmentation and presence of toxocariasis invasion.
Results. As a result of spermograms in the studied groups of men were identified the violations of ejaculate fertility (asteno-, teratoand oligozoospermia). It has been established that the degree disturbance of spermatogenesis (including significant oligoand teratozoospermia) is depending on the fragmentation of DNA in male gametes and the presence of antibodies to toxocara. At the same time more severe patozoospermia observed in the presence of elevated levels of fragmented sperms and the absence of antibodies to toxocara antigens, which is the basis for further research.
Conclusions. Thus, the determination of sperm DNA fragmentation and availability of toxocariasis invasion in men with reproductive disorders is a necessary component of a comprehensive examination, which may allow to improve the diagnostics of male infertility and to promote the development of the right tactics and optimal treatment regimens.
References
Kudlay EN. Faktory muzhskoho besplodyia na sovremennom etape. Zdorov’e Muzhchyny. 2007; 1: 125–128.
Gorpinchenko II. Chelovek v XXI veke. Seksolohycheskye y androlohycheskye aspekty. Zdorov’e Muzhchyny. 2012; 4: 5–18.
Yuzko OM., Yuzko TA., Rudenko NG. Stan ta perspektyvy vykorystannia dopomizhnykh reproduktyvnykh tekhnolohii u likuvanni bezpliddia v Ukraini. Neonatology, Surgery and Perinatal Medicine. 2012; T. ІІ, 4 (6): 26–30.
Abubakirov AN. Povrezhdenye DNK spermatozoydov y muzhskoe besplodye. Urology. 2009; 3: 86–91.
Baikoshkareva SB, Rud SE, Otirbaev MK. Ob yzmenchyvosty eiakuliata. Problems of Reproduction. 2009; 4: 59–61.
Bozhedomov VA, Gromenko DS, Ushakova IV. Okyslytelnyi stress spermatozoydov v patoheneze muzhskoho besplodyia. Urology. 2009; 2: 51–56.
Gleicher N, Barad D. Unexplained infertility: does really exist? Hum Reprod. 2006; 21 (8): 1951–1955.
Bozhedomov VA, Lypatova NA, Sporysh EA [i dr.] Rol’ strukturnykh narushenyi khromatyna DNK spermatozoydov v razvytyy besplodyia. Androlohyia i henytalnaia khyruruhyia. 2012; 3: 83–91.
Calle JF, Muller A, Walschaerts M [el al.] Sperm deoxyribonucleic acid fragmentation as assessed by the sperm chromatin dispersion test in assisted reproductive technology programs: results of a large prospective multicenter study. Fertility and Sterility. 2008; 19 (6): 671–682.
Basil C, Tarlatzis С, Dimitrios G. Goulis. Sperm DNA fragmentation assessment: Is it really helpful? International Society of Gynecological Endocrinology. 2009; 34: 24–27.
Won KY, Kruszon-Moran D, Schantz PM [el al.] National seroprevalence and risk factors for zoonotic Toxocara spp. infection. Am. J. Trop. Med. Hyg. 2008; 79: 552–557.
Kolmogorov VI, Bekysh VYa. Povrezhdenye henoma vladeltsa pri eksperymentalnom toksokaroze i pri sensibilyzatsii belkovym produktom iz tkanei Toxocara canis. Vestnyk VGMU. 2004; 3: 81–89.
World Health Organization (WHO) Laboratory manual for the examination of human semen and sperm-cervical mucus interaction [4th ed.]. New York: Cambridge University Press; 1999: 128.
Rudneva SA, Bragina EE, Aryfulyn EA [i dr.] Frahmentatsyia DNK v spermatozoydakh i ee vzaymosviaz’ s narusheniem spermatogeneza. Andrologyia i genytalnaia khyrurgyia. 2014; 4: 26–33.
