Endothelial factors and blood homocysteine level in adolescents with rheumatic diseases





rheumatic diseases, vascular endothelium function, homocysteine, vascular endothelial growth factor, brain natriuretic peptide, angiotensin-converting enzyme, adolescents


It is known that the dysfunction of the endothelium has crucial role in many pathological conditions and underlines adverse cardiovascular events.

The aim of our study was to determine biologically active substances in the blood that affect endothelial function and homocysteine level in adolescents with rheumatic diseases.

Materials and methods. We examined 68 patients with rheumatic diseases, among them 25 patients with systemic lupus erythematosus (SLE) and 43 patients with juvenile idiopathic arthritis (JIA). Obtained results were compared with similar indicators of peers from the control group. All patients received basic therapy for 12 or more months at the time of examination. Biologically active substances (Homocysteine (Hcy), vascular endothelial growth factor (VEGF), high-sensitivity C-reactive protein (hs-CRP)) were studied by enzyme-linked immunosorbent assay, brain natriuretic peptide (NT-proBNP) by competitive immunoassay, and angiotensin-converting enzyme (ACE) by turbidimetric FAPGG kinetics method.

Results. Patients with rheumatic diseases had a significantly higher level of BNP (p < 0.01). These changes were most significant in patients with SLE. The level of Hcy did not differ from the similar indicator of the control group, but in patients with SLE it was significantly higher (p < 0.01) than in patients with JIA.

Conclusions. In patients with rheumatic diseases, biologically active substances level affecting the endothelium function depends on the disease. Biologically active substances affecting the function of the endothelium were within normal values. Thus, in children with SLE compared with JIA children, an increase in Hcy and NT-proBNP, and a decrease in ACE and hs-CRP protein were found. In children with JIA, normal levels of Hcy and ACE are accompanied by an increase in NT-proBNP and hs-CRP. In adolescents aged 10–18 years with SLE and JIA, multidirectional changes in biologically active substances and homocysteine, affecting the endothelial function of blood vessels were found.

Author Biographies

T. O. Holovko, V. N. Karazin Kharkiv National University, Ukraine

Associate Professor of the Department of Pediatrics; PhD, Head of the Department of Rheumatology and Comorbid Conditions, State Institution “Institute for Children and Adolescents Health Care of the National Academy of Medical Sciences of Ukraine”, Kharkiv

L. F. Bohmat, State Institution “Institute for Children and Adolescents Health Care of the National Academy of Medical Sciences of Ukraine”, Kharkiv

MD, PhD, DSc, Professor, Chief Researcher of the Department of Rheumatology and Comorbid Conditions

N. S. Shevchenko, V. N. Karazin Kharkiv National University, Ukraine

MD, PhD, DSc, Head of the Department of Pediatrics; Leading Researcher of the Department of Rheumatology and Comorbid Conditions, State Institution “Institute for Children and Adolescents Health Care of the National Academy of Medical Sciences of Ukraine”, Kharkiv

Yu. V. Volkova, State Institution “Institute for Children and Adolescents Health Care of the National Academy of Medical Sciences of Ukraine”, Kharkiv

PhD, Head of the Laboratory of Hormonal-Metabolic and Immunological Research

L. L. Sukhova, State Institution “Institute for Children and Adolescents Health Care of the National Academy of Medical Sciences of Ukraine”, Kharkiv

PhD, Researcher of the Laboratory of hormonal-metabolic and immunological research

O. S. Pavlova, V. N. Karazin Kharkiv National University, Ukraine

MD, PhD, Associate Professor, Department of Pediatrics


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How to Cite

Holovko, T. O., Bohmat, L. F., Shevchenko, N. S., Volkova, . Y. V., Sukhova, L. L., & Pavlova, O. S. (2024). Endothelial factors and blood homocysteine level in adolescents with rheumatic diseases. Modern Medical Technology, 16(1), 40–45. https://doi.org/10.14739/mmt.2024.1.298488